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999			_c154919 _d154919
003			OSt
005			20220423153600.0
008			220423b ||||| |||| 00| 0 eng d
082			_a660.6 _bAMR/MO
100			_aAmritha M S
245			_aMolecular characterisation of sweet potato ( Ipomea batatas (L) Lam ) accessions and wild relatives using SSR markers
260			_aVellayani _bDepartment of Plant Biotechnology _c2015
300			_a86 Pages
502			_aMSc
520	3		_aSweet potato (Ipomoea batatas (L.) Lam) is one of the major tuber crops in Asian countries including India, yet the interrelationship and genetic variability among the cultivar and its crop wild relatives remain unclear. The present study utilised 12 simple sequence repeats (SSR) marker for analyse the degree of genetic diversity and relationship within and between 20 sweet potato accessions, 23 wild Ipomoea species and two Merremia species. The SSR primers produced 84 polymorphic alleles within the range of 105-393 bp. That showed an average polymorphism of 94.5%. The average PIC value of 0.67 showed that all the primers were polymorphic in nature. Similarity coefficient based dendrogram and PCA scatter plot clearly differentiated all the cultivars and wild species. This dendrogram helped to identify the close relationship of cultivars with I. triloba and I. trifida at a similarity index of 0.75. The dendrogram based on the SSR molecular data shows high degree of genetic variability within and between hexaploid sweet potato and diploid wild species, whereas lower within the cultivars, which shows that there is a high level of genetic diversity exist within the Ipomoea species. The results supported the current taxonomical position of M. dissecta as Merremia species whereas opposed the taxonomical position of M. vitifolia as Merremia as this study revealed more similarity to Ipomoea than Merremia species. The similarity coefficient varied from 0.37 to 0.96 indicating high variability in the genotypes that were included for the present investigation.
650			_aDepartment of Plant Biotechnology
700			_aMohan C ( Guide )
856			_uhttp://krishikosh.egranth.ac.in/handle/1/5810111196
942			_2ddc _cTH