02899nam a2200193Ia 4500999001700000003000400017005001700021008004100038082002100079100001300100245004300113260012500156502000900281520219900290700002602489856005702515942001202572952012102584  c27177d27177OSt20220305100500.0140128s9999    xx            000 0 und d  a636.0894bPRI/SE  aPriya P   aSeroepidemiology of Bovine Brucellosis  aMannuthybDepartment of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciencesc2003  bMVSc3 aAn investigation was carried out on 1602 cattle and buffaloes of Kerala 
including 1535 cattle and 67 buffaloes to assess the seroprevalence of brucellosis 
using serological tests like Rose Bengal plate' test, Standard tube agglutination 
test, 2-Mercaptoethanol test and Avidin-Biotin ELISA. Sera samples were 
collected from bovines belonging to different farms, hospitals and households of 
Kerala covering eleven districts. Samples were collected randomly from animals 
of different sex, age, breed, gestation, lactation, reproductive performance, 
management practices and also from slaughter house. Out of the 1602 sera 
samples tested 15.29 per cent gave positive result for brucella antibodies. Of this 
cattle showed a seropositivity of 14.72 per cent and buffaloes showed 28.35 per 
cent seropositivity. Female cattle and female buffaloes showed higher 
seroprevalence than male cattle and male buffaloes. Bulls and cows aged more 
than five years showed the highest seropositivity. Among the breeds pure bred 
Holstein Friesian bulls and crossbred Jersey female cattle showed the highest 
seroprevalence. Seroprevalence of brucellosis was found higher in pregnant 
cattle, in late stage of gestation, in pregnant dry animals and also in cows and 
heifers with the history of repeat breeding. Animals maintained under 
unorganised farming system, animals from high altitude agroecological region 
showed more number of positive reactors. Northern zone of Kerala showed more 
number of positive reactors. Districtwise analysis revealed that animals 
belonging to Kozhikode district having highest seropositivity and animals from 
Kottayam and Malappuram districts as zero positives. RBPT, STA T and 2 MET 
was evaluated for the sensitivity, specificity, predictive values and accuracy 
using, A-B ELISA as the 'gold standard'. RBPT was found to have higher 
sensitivity, predictive value of a negative test and accuracy than STAT and 2 
MET and all the three tests found to have 100 per cent specificity and predictive 
value of a positive test with that of ELISA. It was concluded that RBPT can be 
used as a preliminary screening test and ELISA as a confirmatory diagnostic test. 

  aVijayakumar K (Guide)  uhttps://krishikosh.egranth.ac.in/handle/1/5810106493  2ddccTH  00102ddc4070aKAUCLVbKAUCLVcTHESESd2014-03-18l0o636.0894 PRI/SEp172236r2014-03-18 00:00:00w2014-03-18yTH