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Cataloguing and characterization of diseases of ornamental foliage plants in Kerala

By: Contributor(s): Material type: TextPublication details: Vellanikkara Department of Plant Pathology, College of Agriculture 2023Description: xxiv, 204pSubject(s): DDC classification:
  • 632.3 SAN/CA PG
Online resources: Dissertation note: MSc Abstract: Ornamental foliage plants are globally recognised for their brilliant colours, texture, patterns and foliar variegations. They are widely used as an integral component in indoor as well as outdoor gardening and adapt well under low light conditions. The common ornamental foliage plants like Dracaena, Aglaonema and Philodendron have tremendous potential and marketability in the domestic as well as international market which is drastically reduced by different diseases occurring on their foliage. Hence the present study was undertaken to identify various diseases of Dracaena, Aglaonema and Philodendron plants cultivated in Kerala and the pathogens associated with these diseases. A purposive sampling survey was undertaken in ornamental foliage growing areas and commercial nurseries of Wayanad, Malappuram, Thrissur, Ernakulam and Thiruvananthapuram districts representing the Northern, Central and Southern zones of Kerala. From the 11 different survey locations, 18 diseased Dracaena samples, 10 Aglaonema samples and 10 Philodendron samples were collected. Leaf blight and leaf spot were the prominent symptoms observed in these survey locations. The collected samples consisted of 17 leaf blight samples of Dracaena, eight leaf blight samples of Aglaonema and seven leaf blight samples of Philodendron and the leaf spot samples of Dracaena, Aglaonema and Philodendron collected were one, two and three respectively. Among the leaf blights of Dracaena, the sample M Kr DLB2 recorded the highest per cent disease incidence (PDI) and per cent disease severity (PDS) values of 85.00 and 72.22 per cent respectively. The sample, E Ka DLS1 was the only Dracaena leaf spot sample with PDI 18.18 per cent and PDS of 16.66 per cent. Among the Aglaonema samples collected, the leaf blight sample, T Vk ALB2 recorded the highest PDI (37.50 %) and PDS values (20.62 %) and leaf spot sample, E Am ALS2 recorded highest PDI (20.00 %) and PDS (4.51 %). T Vk PLB1 was the Philodendron leaf blight sample with maximum PDI (22.77 %) and PDS (40.00 %). Among the leaf spot samples of Philodendron, severe one was TV Ko PLS1 which recorded PDI of 37.50 and PDS of 7.83 per cent. Isolation and pathogenicity studies resulted in 21 fungal isolates from Dracaena, 13 from Aglaonema and 10 isolates from Philodendron samples. The symptoms associated with these pathogens were studied both under natural as well as under artificial conditions. Genus level identification of each pathogen was carried out on the basis of cultural and morphological characteristics. Based on this the fungal pathogens of Dracaena were identified as Colletotrichum spp. (9 nos.), Fusarium spp. (4 nos.), Lasiodiplodia spp. (3 nos.), Phomopsis spp. (2 nos.) and Neopestalotiopsis sp. The fungal pathogens of Aglaonema were identified as Colletotrichum spp. (7 nos.), Fusarium spp. (2 nos.), Pestalotiopsis sp., Curvularia sp. and Corynespora sp. The pathogenic fungi associated with diseased Philodendron samples were Colletotrichum spp. (8 nos.), Fusarium sp. and Phytophthora sp. Molecular characterization of 13 selected fungal pathogens was attempted by analysing the amplified LSU or ITS sequences of pathogens with NCBI BLASTn database. Based on this the fungal pathogens of Dracaena were Colletotrichum gloeosporioides (Glomerella cingulata) (M Kr DF2), Fusarium oxysporum (M Kr DF4), Diaporthe tulliensis (Phomopsis heveicola) (T Th DF3), Lasiodiplodia theobromae (E Ka DF1) and Neopestalotiopsis aotearoa (TV Vy DF2). The fungal pathogens of Aglaonema, M Ch AF1, T Vk AF2, E Am AF1, E Am AF3 and E Am AF4 were identified upto species level as Pestalotiopsis microspora, Colletotrichum gloeosporioides, Curvularia clavata, Fusarium oxysporum and Corynespora smithii respectively. The result of in silico analysis revealed the fungal isolates of Philodendron spp. T Vk PF1identified as Colletotrichum dracaenophilum, E Am PF3 as Fusarium incarnatum and TV Pu PF1 as Phytophthora nicotianae. Host range studies of 11 fungal pathogens of Dracaena (Colletotrichum spp., Fusarium spp., Neopestalotiopsis sp., Phomopsis spp. and Lasiodiplodia sp.) were carried out on rubber, coconut, nutmeg and banana. All the pathogens produced characteristic symptoms on the detached healthy leaves of the host plants within 1 to 4 days of inoculation except on banana leaves, in which the Colletotrichum spp. (M Kr DF2, T Th DF2, E Ka DF2 and TV Pu DF2) failed to produce any lesions on the inoculated leaves. The in vitro studies using chemical fungicides and biocontrol agents were performed against the 13 selected fungal pathogens of Dracaena, Aglaonema and Philodendron. Mancozeb was the most effective contact fungicide with a per cent inhibition ranging from 77.78 to 100 per cent against all the tested pathogens. Tebuconazole was found most effective systemic fungicide with complete inhibition of the growth of all pathogens. Among the combination fungicides, carbendazim 12% + mancozeb 64% recorded the highest efficiency with complete control of all pathogens at all tested dosages. In vitro studies with different biocontrol agents against selected fungal pathogens revealed PGPM as the most effective with 72.77 to 100 per cent inhibition followed by Trichoderma asperellum (36.66 - 100 %) and PGPR (33.33 - 100 %). The bacterial antagonist, Pseudomonas fluorescens showed the lowest inhibition with a per cent inhibition of 0 to 20.55 per cent. It may be concluded that the present study has enlightened our knowledge of devastating diseases of common ornamental foliage plants cultivated in Kerala viz., Dracaena spp., Aglaonema spp. and Philodendron spp. and also on disease management aspects of the associated pathogens.
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Theses KAU Central Library, Thrissur Theses Thesis 632.3 SAN/CA PG (Browse shelf(Opens below)) Not For Loan 175821

MSc

Ornamental foliage plants are globally recognised for their brilliant colours, texture, patterns and foliar variegations. They are widely used as an integral component in indoor as well as outdoor gardening and adapt well under low light conditions. The common ornamental foliage plants like Dracaena, Aglaonema and Philodendron have tremendous potential and marketability in the domestic as well as international market which is drastically reduced by different diseases occurring on their foliage. Hence the present study was undertaken to identify various diseases of Dracaena, Aglaonema and Philodendron plants cultivated in Kerala and the pathogens associated with these diseases. A purposive sampling survey was undertaken in ornamental foliage growing areas and commercial nurseries of Wayanad, Malappuram, Thrissur, Ernakulam and Thiruvananthapuram districts representing the Northern, Central and Southern zones of Kerala. From the 11 different survey locations, 18 diseased Dracaena samples, 10 Aglaonema samples and 10 Philodendron samples were collected. Leaf blight and leaf spot were the prominent symptoms observed in these survey locations. The collected samples consisted of 17 leaf blight samples of Dracaena, eight leaf blight samples of Aglaonema and seven leaf blight samples of Philodendron and the leaf spot samples of Dracaena, Aglaonema and Philodendron collected were one, two and three respectively. Among the leaf blights of Dracaena, the sample M Kr DLB2 recorded the highest per cent disease incidence (PDI) and per cent disease severity (PDS) values of 85.00 and 72.22 per cent respectively. The sample, E Ka DLS1 was the only Dracaena leaf spot sample with PDI 18.18 per cent and PDS of 16.66 per cent. Among the Aglaonema samples collected, the leaf blight sample, T Vk ALB2 recorded the highest PDI (37.50 %) and PDS values (20.62 %) and leaf spot sample, E Am ALS2 recorded highest PDI (20.00 %) and PDS (4.51 %). T Vk PLB1 was the Philodendron leaf blight sample with maximum PDI (22.77 %) and PDS (40.00 %). Among the leaf spot samples of Philodendron, severe one was TV Ko PLS1 which recorded PDI of 37.50 and PDS of 7.83 per cent. Isolation and pathogenicity studies resulted in 21 fungal isolates from Dracaena, 13 from Aglaonema and 10 isolates from Philodendron samples. The symptoms associated with these pathogens were studied both under natural as well as under artificial conditions. Genus level identification of each pathogen was carried out on the basis of cultural and morphological characteristics. Based on this the fungal pathogens of Dracaena were identified as Colletotrichum spp. (9 nos.), Fusarium spp. (4 nos.), Lasiodiplodia spp. (3 nos.), Phomopsis spp. (2 nos.) and Neopestalotiopsis sp. The fungal pathogens of Aglaonema were identified as Colletotrichum spp. (7 nos.), Fusarium spp. (2 nos.), Pestalotiopsis sp., Curvularia sp. and Corynespora sp. The pathogenic fungi associated with diseased Philodendron samples were Colletotrichum spp. (8 nos.), Fusarium sp. and Phytophthora sp. Molecular characterization of 13 selected fungal pathogens was attempted by analysing the amplified LSU or ITS sequences of pathogens with NCBI BLASTn database. Based on this the fungal pathogens of Dracaena were Colletotrichum gloeosporioides (Glomerella cingulata) (M Kr DF2), Fusarium oxysporum (M Kr DF4), Diaporthe tulliensis (Phomopsis heveicola) (T Th DF3), Lasiodiplodia theobromae (E Ka DF1) and Neopestalotiopsis aotearoa (TV Vy DF2). The fungal pathogens of Aglaonema, M Ch AF1, T Vk AF2, E Am AF1, E Am AF3 and E Am AF4 were identified upto species level as Pestalotiopsis microspora, Colletotrichum gloeosporioides, Curvularia clavata, Fusarium oxysporum and Corynespora smithii respectively. The result of in silico analysis revealed the fungal isolates of Philodendron spp. T Vk PF1identified as Colletotrichum dracaenophilum, E Am PF3 as Fusarium incarnatum and TV Pu PF1 as Phytophthora nicotianae. Host range studies of 11 fungal pathogens of Dracaena (Colletotrichum spp., Fusarium spp., Neopestalotiopsis sp., Phomopsis spp. and Lasiodiplodia sp.) were carried out on rubber, coconut, nutmeg and banana. All the pathogens produced characteristic symptoms on the detached healthy leaves of the host plants within 1 to 4 days of inoculation except on banana leaves, in which the Colletotrichum spp. (M Kr DF2, T Th DF2, E Ka DF2 and TV Pu DF2) failed to produce any lesions on the inoculated leaves. The in vitro studies using chemical fungicides and biocontrol agents were performed against the 13 selected fungal pathogens of Dracaena, Aglaonema and Philodendron. Mancozeb was the most effective contact fungicide with a per cent inhibition ranging from 77.78 to 100 per cent against all the tested pathogens. Tebuconazole was found most effective systemic fungicide with complete inhibition of the growth of all pathogens. Among the combination fungicides, carbendazim 12% + mancozeb 64% recorded the highest efficiency with complete control of all pathogens at all tested dosages. In vitro studies with different biocontrol agents against selected fungal pathogens revealed PGPM as the most effective with 72.77 to 100 per cent inhibition followed by Trichoderma asperellum (36.66 - 100 %) and PGPR (33.33 - 100 %). The bacterial antagonist, Pseudomonas fluorescens showed the lowest inhibition with a per cent inhibition of 0 to 20.55 per cent. It may be concluded that the present study has enlightened our knowledge of devastating diseases of common ornamental foliage plants cultivated in Kerala viz., Dracaena spp., Aglaonema spp. and Philodendron spp. and also on disease management aspects of the associated pathogens.

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