Diversity of coccinellid beetles (Coleoptera: Coccinellidae) of northern Kerala (Record no. 291027)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05412nam a22002177a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.6 |
| Item number | GOK/DI PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Gokul Goutham |
| 245 ## - TITLE STATEMENT | |
| Title | Diversity of coccinellid beetles (Coleoptera: Coccinellidae) of northern Kerala |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Padannakkad |
| Name of publisher, distributor, etc | Department of agricultural entomology, college of agriculture |
| Date of publication, distribution, etc | 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 92,ix, p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | Coccinellid beetles, commonly known as ladybirds or lady beetles, belong to<br/>the family Coccinellidae of order Coleoptera, are species rich and play a significant and<br/>multifaceted role in agriculture. Coccinellids are ecologically and morphologically<br/>diverse and exhibit a wide range of food habits spanning kingdoms and trophic levels.<br/>Most of the coccinellids are predaceous on various agricultural pests, whereas a few are<br/>phytophagous, and some are mycophagous.<br/>The research work on “Diversity of coccinellid beetles (Coleoptera:<br/>Coccinellidae) of northern Kerala” was undertaken in the Department of Agricultural<br/>Entomology, College of Agriculture, Padannakkad during 2021-2023 for the<br/>documentation of the species composition and estimation of diversity indices of<br/>coccinellid beetles in different agro-ecological zones of northern Kerala and to generate<br/>DNA barcodes and carry out molecular analysis of coccinellids associated with<br/>different cropping systems. Purposive surveys were conducted across four different<br/>agro-ecological zones, viz., coastal plain, midland laterites, foothill, and high hill,<br/>covering Kasaragod, Kannur, Kozhikode, and Wayanad districts. Different cropping<br/>systems, viz., rice, vegetables, fruits, and plantation crops were covered during the<br/>survey. A total of 27 species of coccinellids belonging to six tribes were recorded.<br/>Among these, three were phytophagous viz., Afidenta misera, Henosepilachna septima,<br/>and H. vigintioctopunctata and two were mycophagous viz., Illeis bielawskii and Illeis<br/>cincta and rest of 22 species were predacious in nature. Total number of coccinellid<br/>species recorded from rice, vegetables, plantation crops and fruits were seven, 18, 11<br/>and eight, respectively. Among different agroecological zones, high hills recorded<br/>maximum number of coccinellids (20) followed by coastal plain (19), midland laterites<br/>(14) and foothills (10).<br/>Diversity indices to assess species richness, diversity, dominance and evenness<br/>were estimated. The most abundant coccinellid species was H. vigintioctopunctata,<br/>with a relative abundance of 29.86 per cent. The predominant predatory coccinellid was<br/>Cheilomenes sexmaculata (11.88%), followed by Coccinella transversalis (11.72%).<br/>Among various cropping systems the vegetable cropping system displayed highest<br/>species richness, while rice showed lowest richness. High hills showed the highest richness and least rich was foot hills. Species diversity was estimated in terms of<br/>Shannon-Wiener index (H) and the most diverse cropping system was plantation crops<br/>(1.96) and least diverse was rice (1.15) ecosystem. Among the agroecological zones,<br/>high hill (2.47) was the most diverse, and the foothill was the least diverse zone (1.91).<br/>Species dominance was studied by using Simpson’s index. The highest value of<br/>Simpson’s index was observed in rice (0.46) and lowest value in plantation crops (0.17).<br/>Among agroecological zones, coastal plain was least diverse with highest dominance<br/>value (0.21) and lowest dominance value was in midland laterites (0.11). Pielou's<br/>evenness index (J) estimated how evenly species are distributed in a community. The<br/>vegetable cropping system had the lowest species evenness (0.39) while the plantation<br/>crops had the highest (0.65). The species were more evenly distributed in midland<br/>laterites (0.75), while less evenly in coastal plains (0.42).<br/>Total genomic DNA was isolated and mitochondrial Cytochrome Oxidase 1<br/>(CO1) locus was amplified using LCO1490 /HCO2198 primers. DNA sequencing was<br/>done by outsourcing. The forward and reverse COI sequences were trimmed using<br/>BioEdit Sequence Alignment Editor software and assembled using CAP3 sequence<br/>assembler. Molecular analysis of 20 sequences representing 17 species was done using<br/>MEGA11 software. Homology analysis of 14 DNA sequences, encompassing 13<br/>distinct species using Nucleotide BLAST of NCBI revealed a substantial similarity of<br/>over 98 per cent when compared to sequences present in the NCBI database. Six<br/>sequences of four species showed less than 90 per cent similarity to other sequences in<br/>the NCBI database. Phylogenetic trees were constructed using MEGA11 software with<br/>the Maximum likelyhood method and a bootstrap value of 1000. The analysis<br/>incorporated 20 generated sequences in this study and 20 sequences (including Amphix<br/>laevigatus as outgroup) retrieved from the NCBI database. The tree formed two major<br/>clades, one includes I. cincta and all other species formed the second major clade.<br/>Within the second major clade, members of each tribe formed as separate clade except<br/>for S. sp. nr. posticalis. Twenty accessions of 17 species were submitted to NCBI and<br/>BOLD databases<br/> |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Coccinellid beetles |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Agricultural entomology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Coleoptera |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vidya, C V (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810224742 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Item type | Theses |
| 546 ## - LANGUAGE NOTE | |
| Language note | English and Malayalam |
| Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Thesis | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 24/02/2024 | 632.6 GOK/DI PG | 175926 | 24/02/2024 | Theses |