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Bioactivity of essential oil from the tea tree Melaleuca bracteata F. Muell. against stored product pests (Record no. 290660)

MARC details
000 -LEADER
fixed length control field	04361nam a22002057a 4500
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	632.6
Item number	FAR/BI PG
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name	Farzana M Sha
245 ## - TITLE STATEMENT
Title	Bioactivity of essential oil from the tea tree Melaleuca bracteata F. Muell. against stored product pests
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc	Vellayani
Name of publisher, distributor, etc	Department of Agricultural Entomology, College of Agriculture
Date of publication, distribution, etc	2023
300 ## - PHYSICAL DESCRIPTION
Extent	97p.
502 ## - DISSERTATION NOTE
Dissertation note	MSc
520 3# - SUMMARY, ETC.
Abstract	The research work entitled “Bioactivity of essential oil from the tea tree<br/>Melaleuca bracteata F. Muell. against stored product pests” was carried out at the <br/>Department of Agricultural Entomology, College of Agriculture, Vellayani during <br/>2020 to 2022 with an objective to isolate and characterize and evaluate the bioactivity <br/>and persistence of toxicity of M. bracteata essential oil against stored product pests viz. <br/>red flour beetle, Tribolium castaneum (Herbst) and pulse beetle, Callosobruchus <br/>chinensis (Linn.). <br/>The essential oil (EO) from M. bracteata was extracted by hydro distillation in <br/>clevenger type apparatus. Essential oil yield per 100g leaves was observed as 1.08 mL. <br/>The efficacy of tea tree essential oil was tested against the laboratorymaintained culture of T. castaneum and C. chinensis by contact, fumigant and repellent <br/>bioassays and the persistence of toxic activity of the essential oil on the test insects were <br/>also evaluated under laboratory conditions. <br/>In contact toxicity bioassay, the LC50 and LC90 values of the EO against T. <br/>castaneum were 25.16 % and 69.94 %, respectively at 24 hours after treatment (HAT). <br/>The LC50 and LC90 values of EO against C. chinensis were 0.05 % and 2.73 %, <br/>respectively at 24 HAT. In fumigant toxicity bioassay of T. castaneum, the LC50 and <br/>LC90 values of the EO were 7.7mLL-1<br/>and 22.95mLL-1<br/>at 24 HAT. The LC50 and LC90<br/>values of EO against C. chinensis were 4.30 μLL-1<br/>and 29.96 μLL-1<br/>, respectively at 24 <br/>HAT. <br/>In the case of T. castaneum, the maximum repellency observed was 93.75 % at <br/>5% concentration of EO. Cent per cent repellency was not observed here because of the <br/>fumigant effect at higher doses which scattered the released insects into both treated <br/>and untreated filter paper halves in Petri plates. In case of C. chinensis, the insects get <br/>repelled at a very low concentration. 100 per cent repellency was observed at 0.01% <br/>and 0.05% concentration of EO after 2 h and 45 min, respectively.<br/>The toxic effect of M. bracteata EO persisted up to 34 days on C. chinensis<br/>whereas upto 10 days for T. castaneum when exposed to LC90 values obtained from <br/>136<br/>fumigant bioassay. The GC-FID and GC-MS profiling of the EO showed 46 <br/>components where, methyl isoeugenol (69.64%) a phenylpropanoid was found to be <br/>major component followed by methyl cinnamate (8.73%) and methyl eugenol (7.40%), <br/>which may alone or synergistically with other constituents caused the insecticidal <br/>effects. <br/>Experiments on the effect of the EO on the detoxifying enzymes of T. <br/>castaneum and C. chinensis showed that the enzymes like cytochrome P 450, carboxyl <br/>esterase were significantly lower in essential oil treated insects, whereas the activity of <br/>glutathione S- transferase was found to be higher when compared to untreated insects. <br/>Similarly, the total protein content was also found lower in essential oil treated insects <br/>compared to the untreated insects. <br/>In the present investigation, M. bracteata was found to have a good essential oil <br/>yield of 1.08 per cent. The M. bracteata EO showed strong contact, fumigant and <br/>repellent toxicity against C. chinensis when compared to T. castaneum. Against C. <br/>chinensis, the toxic activity of the essential oil persisted up to 34 days. Methyl <br/>isoeugenol (69.53%) was found to be the major component in the essential oil, followed <br/>by methyl cinnamate (8.73%) and methyl eugenol (7.41%), which may alone or <br/>synergistically with other constituents caused the insecticidal effects. The essential oil <br/>in the present investigation has effects on the detoxification enzymes of the test insects.<br/>
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Agricultural Entomology
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Tea tree
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Bioactivity of essential oil
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Melaleuca bracteata F Muell
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Thania Sara Varghese (Guide)
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	https://krishikosh.egranth.ac.in/handle/1/5810224744
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme	Dewey Decimal Classification
Item type	Theses

Holdings
Not for loan	Collection code	Home library	Current library	Shelving location	Date acquired	Full call number	Barcode	Date last seen	Koha item type
Not For Loan	Thesis	KAU Central Library, Thrissur	KAU Central Library, Thrissur	Theses	03/10/2023	632.6 FAR/BI PG	175843	03/10/2023	Theses