Elicitation of phenylpropanoid glycosides biosynthesis and expression profiling of key acteoside biosynthetic genes in Artanema sesamoides Benth (Vathomvaretti) (Record no. 289763)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04542nam a22001937a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | ANU/EL PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Anusha R |
| 245 ## - TITLE STATEMENT | |
| Title | Elicitation of phenylpropanoid glycosides biosynthesis and expression profiling of key acteoside biosynthetic genes in Artanema sesamoides Benth (Vathomvaretti) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Biotechnology, College of Agriculture |
| Date of publication, distribution, etc | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 64p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The study entitled “Elicitation of phenylpropanoid glycoside biosynthesis and<br/>expression profiling of key acteoside biosynthetic genes in Artanema sesamoides Benth<br/>(vathomvaretti)” was carried out during 2019-2021, at the Department of Plant<br/>Biotechnology, College of Agriculture, Vellayani. The objective was to enhance the<br/>phenylpropanoid glycosides synthesis in the callus culture of Artanema sesamoides<br/>using elicitors like salicylic acid, yeast extract and pectin and to study their effect on the<br/>expression of acteoside biosynthetic genes PAL (phenylalanine ammonia-lyase) and HCT<br/>(Hydroxycinnamoyl-CoA Shikimate).<br/>In vitro raised seedlings of A. sesamoides were used for the establishment of<br/>callus culture in MS medium supplemented with 0.5 mgL-1 BA and 0.5 mgL-1 NAA.<br/>Two-week old callus cultures were transferred to liquid MS medium with 0.5 mgL-1<br/>NAA and 0.5 mgL-1 BA and stabilized for 10 days. Elicitors viz., salicylic acid (SA 40<br/>and 100μM), yeast extract (YE 1 and 2 gL-1<br/>) and pectin (100 and 200 mgL-1<br/>) were<br/>added to the suspension culture and incubatedin an orbital shaker (120 rpm) at 24°C for<br/>3 weeks. After elicitation, callus was harvested, dried and finely powdered for analysis.<br/>The spent medium was also analysed for the PPGs.<br/>Phenylpropanoid glycosides (PPGs) were extracted using n-butanol and the<br/>extracts were evaporated to get the crude residues. The total yield of the crude residue of<br/>phenylpropanoid glycosides was maximum (44.88 mgg-1<br/>) in the suspension culture<br/>treated with pectin (200 mgL-1<br/>) and this treatment showed maximum residue (41.6 mgg-1<br/>)<br/>in the callus also. While the additionof pectin increased the accumulation of PPGs in the<br/>callus, the addition of SA and YE increased their exudation in the medium.<br/>Six important PPGs such as acteosides, artanemoside, isoacteoside,<br/>leucosceptoside, martynoside and plantainoside were identified in the butanol extracts<br/>using HPLC. Phenylpropanoid glycosides showed maximum absorbance at 330 nm and<br/>the peaks appeared within the retention time of 40 to 75 min. All the elicitors increased<br/>61<br/>the synthesis of plantinoside. Treatment with SA (40µM) enhanced the content of<br/>martynoside (3.032%) and plantainoside (45.444%) in the callus by 10 and 50 folds<br/>respectively.<br/>In this study, the acteoside content was found to be more in the medium than the<br/>callus. Treatment with (SA 40µM) and YE (2 gL-1<br/>) was found to increase the acteoside<br/>content to a maximum of 10 to 17 folds in the medium. Up to 34.4% increase in the<br/>artanemoside content was observed in the medium with the elicitation by Pectin (100<br/>mgL-1<br/>). Isoacteoside content was increased to 17.3% with elicitation by SA (100 μM).<br/>Real-time PCR was performed to find the effect of elicitors on the expression of<br/>acteoside biosynthesis genes such as PAL and HCT in the callus after 24h and 48h of<br/>elicitation. The quality of cDNA was confirmed by PCR using β-ACTIN gene-specific<br/>primers. The Cq values obtained in RT-qPCR for each gene was further analysed and<br/>relative expression values were obtained using 2-ΔΔCq (Livak) method with ACTIN as the<br/>reference gene.<br/>After 24h of elicitation, 83-fold increase in the expression of PAL gene was<br/>observed in the callus treated with SA (40 μM). Elicitation with YE (1 gL-1<br/>) and Pectin<br/>((100 mgL-1<br/>) showed 8 and 2.3-fold increase in the expression of PAL gene after 24h.<br/>The expression of both PAL and HCT genes was increased up to 2-folds in the callus<br/>treated with SA (100 μM), pectin (100 mgL-1<br/>) and YE (1 gL-1<br/>). After 48h of elicitation,<br/>the expression of PAL and HCT genes was decreased drastically in most of the<br/>treatments.<br/>The study shows a possible use of yeast extract, salicylic acid and pectin for the<br/>in vitro production of phenylpropanoid glycosides from Artanema sesamoides.<br/> |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Biotechnology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Artanema sesamoides Benth |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Phenylpropanoid glycoside |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | K B Soni (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810225520 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Item type | Theses |
| Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 26/07/2022 | 660.6 ANU/EL PG | 175427 | 26/07/2022 | Theses |