Anther culture of Capsicum L. for doubled haploid production (Record no. 289754)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04751nam a22002177a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | NIN/AN PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ninitha Vijayan |
| 245 ## - TITLE STATEMENT | |
| Title | Anther culture of Capsicum L. for doubled haploid production |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Biotechnology, College of Agriculture |
| Date of publication, distribution, etc | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 64p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The study entitled “Anther culture of Capsicum annuum L. for doubled haploid <br/>production” was carried out at the Department of Plant Biotechnology, College of <br/>Agriculture, Vellayani, Thiruvananthapuram during 2019 - 2021. The objective of the study <br/>was to develop haploids of Capsicum annuum var. Arka Meghana by anther culture.<br/>Standardisation of the suitable stage of bud of Capsicum annuum var. Arka Meghana <br/>for anther culture was carried out by staining the anthers of different bud stages with 1% <br/>aceto-orcein and calculating the percentage of microspores at late uninucleate and early <br/>binucleate stages. Buds collected at six days after bud initiation had 57% of microspores in <br/>the late uninucleate stage and 34% in the early binucleate stage. Buds collected at nine days <br/>after bud initiation had 21% of microspores in the late uninucleate stage and 49% in the early <br/>binucleate stage. Hence the buds collected at six and nine days after bud initiation were found <br/>to be most suitable for anther culture.<br/>The optimum surface sterilization condition for buds was standardised by treating the <br/>buds with 70% of ethanol and 4% of sodium hypochlorite for different time intervals. Surface <br/>sterilization with 70% ethanol for 30 seconds followed by 4% sodium hypochlorite for 15 <br/>minutes was found to be most effective.<br/>Anther culture was carried out in nine media compositions for optimization. Anthers <br/>were inoculated onto full strength Murashige and Skoog (1962) medium (MS) and Dumas de <br/>Vaulx (1981) medium (CP) with different concentrations of plant growth regulators Kinetin, <br/>BA, NAA, IAA and 2,4-D with AgNO3 and activated charcoal as additives. The anthers were <br/>incubated at 25°C for two and eight days in darkness. The treatment T5 - MS + 4.00 mg/L <br/>NAA + 0.10 mg/L BA + 0.25% activated charcoal + 10 mg/L AgNO3 incubated at 25°C for <br/>two days in darkness showed callogenesis (3.17%) at six weeks after inoculation. All the <br/>treatments incubated at 25°C for eight days in darkness showed response at sixth week of <br/>inoculation with callogenesis varying from 3.75% to 15.38%. Among the nine treatments, <br/>highest callogenesis of 15.38% was observed in the treatment T5 at sixth week of inoculation. <br/>Globular embryo initiation (1.25%) was observed in the treatment T4 - MS + 4.00 mg/L NAA <br/>+ 1.00 mg/L BA at sixth week of inoculation.<br/>The six treatments that showed good response at 25°C incubation temperature and <br/>eight days darkness were incubated at 35°C in two days darkness. All the treatments showed <br/>response varying from 4% to 15.54% of callogenesis at second week of inoculation. <br/>Callogenesis varying from 10.66% to 34.48% and embryonic calli induction varying from <br/>3.17% to 17.24% was observed in the fourth week of inoculation. At sixth week of <br/>inoculation callogenesis varied from 11.11% to 37.93% and embryonic callus induction <br/>varied from 3.17% to 19.54%. Among the six treatments, the maximum callogenesis of <br/>37.93% and embryonic calli induction of 19.54% was observed in the treatment T5 at fourth <br/>week of inoculation. <br/>Embryogenesis was observed in the treatment T7 – MS + 4.00 mg/L NAA + 0.50 <br/>mg/L BA+ 0.25% activated charcoal + 15 mg/L AgNO3 (1.82%) and treatment T6 - MS + <br/>4.00 mg/L NAA + 0.10 mg/L BA + 0.25% activated charcoal + 15mg/L AgNO3 (1.33%) at <br/>fourth week of inoculation. <br/>At sixth week of inoculation, embryo induction (1.15%) was observed in the <br/>treatment T5 – MS + 4.00 mg/L NAA + 0.10 mg/L BA + 0.25% activated charcoal + 10mg/L <br/>AgNO3. Highest embryogenesis of 2.72% was observed in the treatment T7 – MS + 4.00 <br/>mg/L NAA + 0.50 mg/L BA+ 0.25% activated charcoal + 15 mg/L AgNO3. <br/>To conclude, among the treatments tested in Capsicum annuum var. Arka Meghana <br/>the best treatment for indirect embryogenesis and direct embryogenesis was MS + 4.00 mg/L <br/>NAA + 0.10 mg/L BA + 0.25% activated charcoal + 10 mg/L AgNO3 and MS + 4.00 mg/L <br/>NAA + 0.50 mg/L BA + 0.25% activated charcoal + 15 mg/L AgNO3 respectively at culture <br/>conditions of 35°C initial incubation temperature and two days darkness. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Biotechnology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Capsicum |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Anther culture |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Arka Meghana |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Embryogenesis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Swapna Alex (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810225389 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Item type | Theses |
| Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 25/07/2022 | 660.6 NIN/AN PG | 175425 | 25/07/2022 | Theses |