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Efficacy of argyrrophilic nucleolar organizer region count test for in vivo biomaterial evaluation (Record no. 26725)

MARC details
000 -LEADER
fixed length control field	02834nam a2200181Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field	OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field	20221014101327.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field	140128s9999 xx 000 0 und d
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	636.089
Item number	UMA/EF
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name	Umashankar PR
245 ## - TITLE STATEMENT
Title	Efficacy of argyrrophilic nucleolar organizer region count test for in vivo biomaterial evaluation
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc.	Mannuthy
Name of publisher, distributor, etc.	Centre of Excellence in Pathology, College of Veterinary and Animal Sciences
Date of publication, distribution, etc.	2001
502 ## - DISSERTATION NOTE
Degree type	MVSc
520 3# - SUMMARY, ETC.
Summary, etc.	The efficacy of argyrophilic Nucleolar organizer Region (AgNOR) count test in the evaluation of biomaterial was assessed in this study utilizing a reactive (medium chromic catgut) and a non-reactive (Polypropylene) material.<br/><br/> The materials were implanted into rat gluteus muscle for a duration of seven and 14 days and sham surgery done on a separate group of animals served as the control.<br/><br/> The tissue response such as inflammation, muscle degeneration and peri-implant scar thickness around the reactive and non-reactive implant and the controls at seven and 14 days were studied using H&E and special stains for collagen. Besides this the estimation of collagen of the reactive and non-reactive material implanted tissue was studied. The observations made in this were compared with the fibroblast proliferation response at the respective durations as assessed by the AgNOR count estimation.<br/><br/> On histopathological evaluation, the reactive material showed an initial acute inflammatory response with mild fibrosis and collagen deposition which subsequently settled into a chronic form with severe fibrosis and more collagen deposition at the end of 14 days. Non-reactive material elicited an acute inflammation initially (seven days post implantation) which settled into a mild chronic response at 14 days post implementation. Mild fibrosis and less collagen deposition were noticed in the peri-implant area at both the duration. Observations on fibroblast proliferation as assessed by AgNOR count test well correlated to the above findings. The AgNOR counts observed with reactive material at seven days and 14 days were 1.97 + 0.03 and 2.23 + 0.02 respectively. The counts for non-reactive material were 1.81 + 0.01 and 1.67 + 0.02 at seven and 14 days post implantation respectively.<br/><br/> The collagen content of the reactive and non-reactive implanted tissue did not reveal any statistically significant difference as compared with the respective control. From this investigation it was proved that AgNOR count test could be reliably applied for biomaterial evaluation even at seven days duration and it formed a valuable adjunct to the routine method employed in the in vivo evaluation of biomaterials.<br/>
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Divakaran Nair N (Guide)
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	<a href="https://krishikosh.egranth.ac.in/handle/1/5810153842">https://krishikosh.egranth.ac.in/handle/1/5810153842</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme	Dewey Decimal Classification
Koha item type	Theses

Holdings
Withdrawn status	Lost status	Source of classification or shelving scheme	Damaged status	Not for loan	Home library	Current library	Shelving location	Date acquired	Total Checkouts	Full call number	Barcode	Date last seen	Price effective from	Koha item type
		Dewey Decimal Classification			KAU Central Library, Thrissur	KAU Central Library, Thrissur	Theses	18/03/2014		636.089 UMA/EF	171784	18/03/2014	18/03/2014	Theses