Enhancement of phenyl propanoid glycosides in artanema sesamoides benth (vathomvaretti)by hairy root induction (Record no. 163815)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 04102nam a22001697a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | ELI/EN |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Elizebeth Jose |
| 245 ## - TITLE STATEMENT | |
| Title | Enhancement of phenyl propanoid glycosides in artanema sesamoides benth (vathomvaretti)by hairy root induction |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Biotechnology, College of Agriculture |
| Date of publication, distribution, etc | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 79p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The study entitled “Enhancement of phenyl propanoid glycosides in<br/>Artanema sesamoides Benth (vathomvaretti) by hairy root induction” was<br/>conducted at the Department of Plant Biotechnology, College of Agriculture,<br/>Vellayani and Aromatic and Medicinal Plants Research Station, Odakkali during<br/>2016-2018. The objective of the study was to scale up the production of phenyl<br/>propanoid glycosides in Artanema sesamoides Benth by inducing hairy roots<br/>using Agrobacterium rhizogenes.<br/>The explants for the Agrobacterium rhizogenes mediated transformation<br/>were obtained from the in vitro raised seedlings of A. sesamoides. Seeds collected<br/>from AMPRS, Odakkali were germinated in basal Murashige and Skoog (MS)<br/>and half MS media. Maximum seed germination was observed in basal MS<br/>medium with 94 per cent seed germination. Hundred per cent multiple shoot<br/>induction was observed in basal MS medium and in MS medium supplemented<br/>with Benzyladenine (BA) 0.1 mg L-1 using node as explant. Maximum number of<br/>shoots (21 nos) per explant was seen with BA 0.1 mg L-1. Addition of BA at >0.2<br/>mg L-1 resulted in green undifferentiated mass of cells.<br/>Callus induction was tried in explants like node, leaf and root in basal MS<br/>medium supplemented with combinations of growth regulators like BA, NAA,<br/>IAA and 2,4-D. Node and root produced calli in combinations of BA with NAA<br/>or IAA and their response was better compared to leaf. MS medium supplemented<br/>with 2, 4-D or BA did not produce any callus.<br/>Hairy root induction in A. sesamoides was attempted with A. rhizogenes<br/>strains, MTCC 2364, MTCC 532 and ATCC 15834. A. sesamoides callus was<br/>found insensitive to cefotaxime (bacteriostatic agent) upto 500 mg L-1. Among the<br/>different explants tried for hairy root induction, calli infected with MTCC 2364<br/>(Infection for 15 and 20 minutes and co-cultivation period of 48 h) and MTCC<br/>532 (Infection for 15, 20 and 30 minutes and co-cultivation period of 48 h)<br/>showed 100% survival on MS medium supplemented with cefotaxime (200 mg L-<br/>1<br/>). They also showed regeneration to produce shoots and roots in two weeks. No<br/>hairy root formation was observed in any of the treatments. The in vitro roots<br/>obtained were analysed for the presence of rol gene using PCR and no<br/>amplification was found.<br/>Phenyl propanoid glycosides were quantified using HPLC with the help of<br/>class LC 10 software (Shimadzu, Japan) with PDA detector. Peaks related to<br/>acteoside, artanemoside A, isoacteoside, leucoseptoside A and martynoside were<br/>identified and quantified from roots of field grown (in vivo) plants, in vitro roots<br/>and callus. Callus showed the highest quantity of all the five phenyl propanoid<br/>glycosides, followed by in vivo roots and in vitro roots.<br/>Economics analysis showed that the cost of production per gram dry<br/>weight of the callus was found to be less compared to that of in vivo roots. Since<br/>phenyl propanoid glycosides content in the callus is more than the in vivo root,<br/>large scale production of the compound is economically feasible in in vitro<br/>conditions.<br/>In the present study, in vitro multiplication of A. sesamoides is reported for<br/>the first time. Hairy roots could not be produced in this study, which may be due<br/>to the non virulence of the A. rhizogenes strains used or the presence of inhibitory<br/>substances in the explant, which needs further investigation. Increased phenyl<br/>propanoid glycosides content in callus indicates the scope of using an in vitro<br/>system for the continuous production of these compounds.<br/> |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sony, K B (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810145855 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Item type | Theses |
| Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 20/02/2019 | 660.6 ELI/EN | 174402 | 20/02/2019 | Theses |