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Novel bioformulations of entomopathogenic fungi and their efficacy aganist banana weevils (Record no. 161809)

MARC details
000 -LEADER
fixed length control field	05286nam a2200169 4500
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	632.6
Item number	REM/NO
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name	Remya S
245 ## - TITLE STATEMENT
Title	Novel bioformulations of entomopathogenic fungi and their efficacy aganist banana weevils
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc	Vellayani
Name of publisher, distributor, etc	Department of Agricultural Entomology, College of Agriculture
Date of publication, distribution, etc	2018
300 ## - PHYSICAL DESCRIPTION
Extent	123p.
502 ## - DISSERTATION NOTE
Dissertation note	MSc
520 3# - SUMMARY, ETC.
Abstract	The study entitled ‘Novel bioformulations of entomopathogenic fungi and<br/>their efficacy against banana weevils’ was carried out at Dept. of Agrl.<br/>Entomology, College of Agriculture, Vellayani during 2016-2018, with the<br/>objective<br/>to<br/>develop<br/>novel<br/>formulations<br/>of<br/>entomopathogenic<br/>fungi,<br/>Metarhizium anisopliae (Metch.) Sorokin, Beauveria bassiana (Bals.) Vuillemin<br/>and to evaluate their efficacy in managing banana weevils. It was intended to<br/>develop capsule and gel formulations.<br/>Experiment to standardize a coating material for developing capsules revealed<br/>that Hard Gelatin Transparent (HGT), Hard Gelatin Coloured (HGC) and<br/>Hydroxy Propyl Methyl Cellulose (HPMC), were equally stable under ambient<br/>conditions of storage (26 -33°C and RH 60-80%). On testing their ability to<br/>disintegrate under field conditions, it was noted that chitosan filled HGT capsules<br/>easily disintegrated at 20% soil moisture after 36 h and after 24 h at 30 % soil<br/>moisture. It took 144 h for all capsules to disintegrate in sheath or leaf axil and 24<br/>h in bore holes. Talc and chitosan were superior, in maintaining storage stability<br/>as well as degradability.<br/>A trial conducted to determine the moisture content of capsules, revealed that<br/>10 % was the ideal moisture level content of the ingredient, to maintain storage<br/>stability as well as viability of the formulation. Viability noticed after three months<br/>was 2.29 × 107 cfu mL-1 and 2.27 × 107 cfu mL-1 in chitosan based capsules of<br/>M. anisopliae and B. bassiana and 2.10 × 107 cfu mL-1 and 0.76 × 107 cfu mL-1 in<br/>talc based capsules. Therefore, capsules were developed with HGT coating, with<br/>chitosan / talc as carrier at 10 % moisture content.<br/>Shelf life studies revealed that chitosan was the best carrier material<br/>compared to talc in retaining viability of Metarhizium capsules, with a mean cfu<br/>of 2.51 × 107 mL-1 and 1.77× 107 mL-1. During a period of three months of<br/>storage, there was no decline in the number of viable colonies, cfu ranging from<br/>1.72 × 107 mL-1 to 2.79 × 107 mL-1. . Storage under refrigeration had better<br/>retention of viability (2.63 × 107 cfu mL-1) than at room temperature (1.64× 107<br/>cfu<br/>mL-1). In Beauveria capsules, the viability did not differ in both chitosan<br/>and talc based capsules. Here also, the number of viable colonies did not decline<br/>statistically, till three months of storage, with mean cfu values ranging from 1.85<br/>× 107 mL-1 to 2.36 × 107 mL-1. In general, shelf life of capsules could be extended<br/>by two months when chitosan was used as the carrier material. Low temperature<br/>storage could also improve the shelf life by two months.<br/>Shelf life studies of gel formulations indicated that both Metarhizium and<br/>Beauveria gels exhibited high viability in chitosan at room temperature and under<br/>refrigeration. The mean number of viable colonies observed was 2.39 × 106 cfu<br/>mL-1 in chitosan based gels of Metarhizium and 2.17 × 106 cfu mL-1 in Beauveria<br/>gels. The number of viable colonies of Metarhizium and Beauveria observed<br/>throughout the experimental period did not vary significantly over three months of<br/>storage. The mean number of cfu being 2.26 × 107 mL-1 on the 15th day and 1.41 x<br/>106 mL-1 on the 90th day . It was also inferred that chitosan when used for<br/>formulating gels could extend the shelf life of both organisms by one month.<br/>Pathogenicity test disclosed the affinity of M. anisopliae to rhizome<br/>weevil and B. bassiana to pseudostem weevil. Pot culture studies to evaluate<br/>chitosan and talc based capsules of B. bassiana revealed that both the capsules of<br/>Beauveria were effective as chlorpyriphos 20 EC 0.05% for pseudostem weevil, in<br/>prophylactic and curative methods. The damage index (DI) was one each and<br/>reduction in pest population was 91.67 % in prophylactic control and 91.67-100 %<br/>in curative control. Metarhizium capsules reduced the damage caused by rhizome<br/>significantly, but the reduction in pest population was only to the tune of 47-55 %<br/>in prophylactic and 50- 58 % in curative control. Chitosan based gel of<br/>Metarhizium tested against rhizome weevil, reduced the damage significantly<br/>(DI 46.67 and 30, in prophylactic and curative methods). The pest population was<br/>reduced by 61.11 % in prophylactic and 36.11 % in curative methods.<br/>The study could standardize the protocol for capsule and gel formulations<br/>of entomopathogenic fungi, retaining the viability and infectivity upto three<br/>months of storage. It is concluded that placement of Beauveria capsules in leaf<br/>axils prophylactically and curatively can effectively control pseudostem weevil and<br/>Metarhizium capsules and gels placed in the rhizosphere could manage the rhizome<br/>weevil moderately.<br/>
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Agricultural Entomology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Reji Rani O P (Guide)
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	https://krishikosh.egranth.ac.in/handle/1/5810154464
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme	Dewey Decimal Classification
Item type	Theses

Holdings
Not for loan	Collection code	Home library	Current library	Shelving location	Date acquired	Full call number	Barcode	Date last seen	Koha item type
Not For Loan	Reference Book	KAU Central Library, Thrissur	KAU Central Library, Thrissur	Theses	22/09/2018	632.6 REM/NO	174288	22/09/2018	Theses