Enhancement of resistance to bacterial wilt in tomato by endophytic microbial communities (Record no. 141721)
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| fixed length control field | 03728nam a22002177a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220418154530.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151109b xxu||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.3 |
| Item number | DEE/EN |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Deepa James |
| 245 ## - TITLE STATEMENT | |
| Title | Enhancement of resistance to bacterial wilt in tomato by endophytic microbial communities |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellanikkara |
| Name of publisher, distributor, etc. | Department of plant pathology, College of horticulture |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 148 Pages |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | PhD |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The study on “Enhancement of resistance to bacterial wilt in tomato by<br/>endophytic microbial communities” was conducted in the Department of Plant<br/>Pathology, Department of Agricultural Microbiology, and Centre for Plant<br/>Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara during<br/>the period 2011-2014.<br/>The endophytes were isolated from root and stem of healthy tomato plants<br/>from 16 locations of north, central and south Kerala. Endophytic microbial<br/>population varied with the plant samples and the population was more in root than<br/>stem. Bacterial population was higher than fungi and actinomycetes in root and stem.<br/>Among 154 endophytes isolated, 12 out of 79 bacteria, 16 out of 68 fungi, and four<br/>out of seven actinomycetes were antagonistic to R. solanacearum in in vitro. Among<br/>them, five bacteria, eight fungi, and two actinomycetes were promising in planta.<br/>Mutually compatible endophytic isolates were selected for the development of<br/>consortia and these were identified based on cultural, morphological and molecular<br/>characters. Of the five consortia tested, the one consisted of Trichoderma viride-1,<br/>T. viride-2, T. harzianum-1, Bacillus subtilis, and Streptomyces thermodiastaticus<br/>showed effect in reducing wilt incidence.<br/>Comparative study of the microbial consortium with individual endophytes<br/>showed the higher efficacy of consortium in reducing the wilt incidence. The<br/>endophytes were reisolated from soil, root, and stem of tomato plants. In pot culture<br/>experiment, the consortium applied as seed treatment + seedling dip + soil application<br/>at 45 DAP showed the minimum wilt incidence. Studies on the mechanism of<br/>antagonism of endophytic isolates showed, positive reaction towards ammonia and<br/>negative for HCN and siderophore production. The volatile and nonvolatile<br/>metabolites of the endophytes inhibited the pathogen. The endophytes showed<br/>varying levels of IAA and salicylic acid production with the maximum in<br/>T. harzianum-1 and B. subtilis respectively.<br/>Study on the effect of secondary metabolites of endophytes on the disease<br/>indicated that, seedlings dipped first in culture filtrate for 2 h and later dipped in<br/>bacterial inoculum for 30 min before planting showed the lowest per cent wilt<br/>incidence with the minimum for T. viride-2. Induction of systemic resistance was<br/>studied by assay of defense related compounds such as phenols, oxidative enzymes<br/>and PR proteins. The plants treated with microbial consortium showed higher activity<br/>of the defense related compounds with the maximum in plants inoculated with both<br/>consortium and pathogen.<br/>Field evaluation of endophytic consortium against bacterial wilt showed<br/>reduction in wilt incidence of 40.85 per cent in highly susceptible variety, PKM-1,<br/>46.94 per cent in susceptible F1 hybrid, COTH-3, and 52.81 per cent in moderately<br/>resistant variety, Mukthi.<br/>Thus, the study revealed that, the application of endophytic consortium can<br/>enhance the resistance mechanism in tomato against bacterial wilt pathogen,<br/>R. solanacearum. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant pathology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Tomato |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sally K Mathew (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://krishikosh.egranth.ac.in/handle/1/5810109626">http://krishikosh.egranth.ac.in/handle/1/5810109626</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Theses |
| Withdrawn status | Lost status | Damaged status | Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 21/10/2015 | 632.3 DEE/EN | 173477 | 21/10/2015 | 21/10/2015 | Theses |